Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells

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dc.contributor.affiliationPontificia Universidad Católica del Perú. Departamento de Ciencias
dc.contributor.authorLopez, J.M.
dc.date.accessioned2026-03-13T16:59:04Z
dc.date.issued2023
dc.description.abstractStudies of intrinsically disordered proteins (IDPs) under physiological conditions by conventional NMR methods based on proton detection are severely limited by fast proton amide solvent exchange. Carbon detection has been proposed as a solution to the exchange problem but is hampered by low sensitivity. Here, we present a protocol combining proton–nitrogen cross-polarization and carbonyl detection to record high-resolution and high-sensitivity NMR spectra of IDPs under physiological conditions. The protocol describes a step-by-step method to register high-quality N-CO correlation spectrum of alpha-synuclein in E.coli bacterial cells at 37 °C.
dc.description.sponsorshipFunding: The author wants to thank all the people involved in the development of this method: Dr. Robert Schneider, Dr. Francois-Xavier Cantrelle, Dr. Isabelle Huvent, and Dr. Guy Lippens. The methods here presented were funded by the Centre National de la Recherche Scióntifique, a LABEX DISTALZ grant (Development of iónovative Strategies for a Transdisciplinary Approach to Alzheimer’s Disease), and the CNRS Large Scale Facility NMRTHC Fr3050. The author acknowledges Consejo Nacional de Ciencia, Tecnolo-gía e Innovación Tecnológica (008-2017-FONDECYT) for the partial support provided during the preparatión of this manuscript.
dc.identifier.doihttps://doi.org/10.1007/978-1-0716-2597-2_28
dc.identifier.urihttp://hdl.handle.net/20.500.14657/206152
dc.language.isoeng
dc.publisherHumana Press Inc.
dc.relation.ispartofurn:isbn:978-1-0716-2597-2
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.sourceMethods in Molecular Biology; Vol. 2551 (2023)
dc.subjectChemistry
dc.subjectNuclear magnetic resonance spectroscopy
dc.subjectProton
dc.subjectAmide
dc.subjectHigh resolution
dc.subjectPolarization (electrochemistry)
dc.subjectSpectroscopy
dc.subjectSolvent
dc.subjectAnalytical Chemistry (journal)
dc.subjectPhysical chemistry
dc.subjectChromatography
dc.subjectStereochemistry
dc.subjectOrganic chemistry
dc.subjectPhysics
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#1.04.04
dc.titleCombined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells
dc.typehttp://purl.org/coar/resource_type/c_3248
dc.type.otherCapítulo de libro
dc.type.versionhttps://vocabularies.coar-repositories.org/version_types/c_970fb48d4fbd8a85/

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