Backbone chemical shift assignment of macrophage infectivity potentiator virulence factor of Trypanosoma cruzi

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dc.contributor.affiliationPontificia Universidad Católica del Perú. Departamento de Ciencias
dc.contributor.authorLopez, J.M.
dc.contributor.authorAntiparra, R.
dc.contributor.authorLippens, G.
dc.contributor.authorZimic, M.
dc.contributor.authorSheen, P.
dc.contributor.authorMaruenda, H.
dc.date.accessioned2026-03-13T16:58:04Z
dc.date.issued2019
dc.description.abstractChagas disease is a trypanosomiasis disease inflicted by Trypanosoma cruzi parasite. In Latin America, at least 10 million people are infected and annually, 10,000 casualties are deplored. Macrophage infectivity potentiator protein is one of the major virulence factors secreted by T. cruzi (TcMIP) in order to infect its host but little is known about its mechanism of action. Studies confer TcMIP an important role in the extracellular matrix transmigration and basal lamina penetration. Here, we report the backbone 1H, 13C, and 15N resonance assignment of TcMIP and the comparison of the secondary structure obtained against reported X-ray crystallography data.
dc.description.sponsorshipFunding: Fig. 3 Left, TcMIP X-ray structure (PDB: 1JVW), label in red unassigned residues. Right, plot of assigned chemical shift vs. predicted chemical shift. Predicted shifts were obtained from X-ray structure (PDB: 1JVW) using SHIFTX2 (Han et al. 2011) Acknowledgements This work was funded by ICGEB early career grant (CRP/PER16-05-EC), Consorcio de Universidades (PIIM-2016), Programa Innóvate Perú del Ministerio de la Producción (203-INNOVATEPERU-EC-2016) and Cienciactiva - CONCYTEC (008-2017-FONDECYT). NMR access to the 800MHz spectrometer was granted by MetaToul (Toulouse metabolomics & fluxomics facilities, www.metatoul.fr) as part of the French National Infrastructure for Metabolomics and Fluxomics MetaboHUBAR-11-INBS-0010 (www.metabohub.fr), supported by the Région Occitanie, the ERDF, the SICOVAL, and the French Minister of Education & Research.; Funding text 2: This work was funded by ICGEB early career grant (CRP/PER16-05-EC), Consorcio de Universidades (PIIM-2016), Programa Inn?vate Per? del Ministerio de la Producci?n (203-INNOVATEPERU-EC-2016) and Cienciactiva - CONCYTEC (008-2017-FONDECYT).?NMR access to the 800MHz spectrometer was granted by MetaToul (Toulouse metabolomics & fluxomics facilities, www.metatoul.fr) as part of the French National Infrastructure for Metabolomics and Fluxomics MetaboHUBAR- 11-INBS-0010 (www.metabohub.fr), supported by the Re?gion Occitanie, the ERDF, the SICOVAL, and the French Minister of Education & Research.
dc.identifier.doihttps://doi.org/10.1007/s12104-018-9844-1
dc.identifier.urihttp://hdl.handle.net/20.500.14657/205758
dc.language.isoeng
dc.publisherSpringer Netherlands
dc.relation.ispartofurn:issn:1874-2718
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.sourceBiomolecular NMR Assignments; Vol. 13, Núm. 1 (2019)
dc.subjectMacrophage infectivity potentiator
dc.subjectTcMIP
dc.subjectVirulence factor
dc.subjectNMR resonance assignment
dc.subjectTrypanosoma cruzi
dc.subjectProlyl cis trans isomerase
dc.subjectFK506 binding protein
dc.subjectFKBP-like protein
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#1.06.03
dc.titleBackbone chemical shift assignment of macrophage infectivity potentiator virulence factor of Trypanosoma cruzi
dc.typehttp://purl.org/coar/resource_type/c_6501
dc.type.otherArtículo
dc.type.versionhttps://vocabularies.coar-repositories.org/version_types/c_970fb48d4fbd8a85/

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